Human Endothelial Cells Synthesize Protein
نویسندگان
چکیده
Human umbilical vein endothelial cells were analyzed for the presence of prothrombin. factor VII, protein C, and protein S in culture supernatants and cell extracts using specific radioimmunoassays. Only protein S was detected. Conditioned medium from 24-hour cultures and cell lysates contained 21.7 ng/mL and 88.8 ng/107 cells of protein S, respectively. Intrinsic labeling and immunoprecipitation indicated that protein S was synthesized and secreted as a 75,000 molecular weight protein. Vitamin K, phorbol myristate acetate, and thrombin increased the production or specific activity (determined from activity/antigen ratios of 0.99 to 1 .07, 0.93 to 1 .04, and 0.90 to 1 .04, respectively) of
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